| Lewis Wilson | Batman/Bruce Wayne |
| Douglas Croft | Robin/Richard 'Dick' Grayson |
| J. Carrol Naish | Dr. Tito Daka |
| Shirley Patterson | Linda Page |
| Dick Curtis | Agent Croft of Section 50 [Chs. 10-11] |
| Lester Dorr | Henchman Lawson [Chs. 10-11] |
| Kenne Duncan | Fred, the Mechanic [Chs. 5-6] |
| Robert Fiske | Henchman Foster [Chs. 1-4] |
| Sam Flint | Dr. G. H. Borden [Ch. 1] |
| Jerry Frank | Cave of Horrors Thug |
| Terry Frost | Male nurse [Ch. 7] |
| Gus Glassmire | Martin Warren |
| Karl Hackett | Wallace |
| Earle Hodgins | Joe, the Cave of Horrors Barker |
| Frank Austin | Hotel Desk Clerk [Ch. 2] |
| William Austin | Alfred Beagle |
| Mauritz Hugo | Doctor [Ch. 3] |
| Jack Ingram | Henchman Klein [Chs. 5-14] |
| Warren Jackson | Bernie, Owner of Sphinx Club |
| I. Stanford Jolley | Henchman Brett [Chs. 2-5] |
| Eddie Kane | J. Hanson, Bail Bondsman [Ch. 11] |
| George J. Lewis | Henchman Burke [Chs. 5-14] |
| Tom London | Henchman Andrews [Chs. 8-14] |
| George Magrill | Henchman at Sphinx Club [Ch. 9] |
| Knox Manning | Narrator |
| John Maxwell | Sam Fletcher |
| Charles Middleton | Ken Colton [Chs. 6-9] |
| Pat O'Malley | Policeman [Chs. 6, 15] |
| Ted Oliver | Marshall |
| Bud Osborne | Brown, Zombie [Chs. 14-15] |
| Gary Owens | Narrator (1966 re-release) |
| Stanley Price | Henchman Driver [Chs. 1-2] |
| Frank Shannon | Dr. Hayden [Ch. 1] |
| Michael Vallon | Preston |
| Anthony Warde | Henchman Stone [Chs. 2-4] |
| Charles C. Wilson | Police Captain Arnold |
| Lynton Brent | Jim Bramwell, Mechanic [Chs. 5-6] |
| George Chesebro | Henchman Brennan [Chs. 1-5] |