| Jeffe Alperi | Policeman ("Lumiere and Company") |
| Robert Chadwick | Father ("The Grandmother") |
| Catherine E. Coulson | Amputee ("The Amputee") |
| Eddy Dixon | Rock-a-billy Guy ("The Cowboy and the Frenchman") |
| Frederic Golchan | Pierre the Frenchman ("The Cowboy and the Frenchman") |
| Rick Guillory | Howdy ("The Cowboy and the Frenchman") |
| Michael Horse | Broken Feather ("The Cowboy and the Frenchman") |
| Patrick Houser | Gun Twirler ("The Cowboy and the Frenchman") |
| Stan Lothridge | Policeman ("Lumiere and Company") |
| David Lynch | Himself - Narrator |
| Peggy Lynch | Girl ("The Alphabet") |
| Virginia Maitland | Mother ("The Grandmother") |
| Dorothy McGinnis | Grandmother ("The Grandmother") |
| Jack Nance | Pete ("The Cowboy and the Frenchman") |
| Russ Pearlman | Dead Son ("Lumiere and Company") |
| Pam Pierrocish | Mother ("Lumiere and Company") |
| Clyde Small | Father ("Lumiere and Company") |
| Talisa Soto | French Girl ("The Cowboy and the Frenchman") |
| Harry Dean Stanton | Slim ("The Cowboy and the Frenchman") |
| Tracey Walter | Dusty ("The Cowboy and the Frenchman") |
| Richard White | Mike ("The Grandmother") |
| Mark Wood | Policeman ("Lumiere and Company") |
| Catherine E. Coulson | Woman with amputation to the legs |
| David Lynch | Unable and scared nurse |
| Richard White | Boy |
| Dorothy McGinnis | Grandmother |
| Virginia Maitland | Mother |
| Robert Chadwick | Father |
| Peggy Lynch | Girl |
