| Boris Karloff | Imhotep/Ardath Bey |
| Zita Johann | Helen Grosvenor/Princess Anckesen-Amon |
| David Manners | Frank Whemple |
| Arthur Byron | Sir Joseph Whemple |
| Edward Van Sloan | Dr. Muller |
| Bramwell Fletcher | Ralph Norton |
| Noble Johnson | The Nubian |
| Kathryn Byron | Frau Muller |
| Leonard Mudie | Prof. Pearson |
| James Crane | The Pharaoh |
| Henry Victor | The Saxon warrior |
| Arnold Gray | Knight |
| Leyland Hodgson | Gentleman #2 |
| Eddie Kane | Dr. LeBarron |
| Tony Marlow | Police inspector |
| C. Montague Shaw | Gentleman #1 |
| Pat Somerset | Helen's dancing partner |
| Arthur Tovey | Bit part (Nubian) |
| Dick Foran | Stephen Banning |
| John Hubbard | Dr. John Banning |
| Elyse Knox | Isobel Evans |
| George Zucco | Andoheb |
| Wallace Ford | 'Babe' Hanson |
| Turhan Bey | Mehemet Bey |
| Virginia Brissac | Mrs. Ella Evans |
| Cliff Clark | Sheriff |
| Mary Gordon | Jane Banning |
| Paul E. Burns | Jim, the Banning caretaker |
| Frank Reicher | Prof. Matthew Norman |
| Emmett Vogan | Coroner |
| Lon Chaney Jr | .Kharis, the mummy |
| James Crane | (stock footage from 'The Mummy')) |
| Frank Darien | Old Townsman |
| Jimmy Fairfax | Ship's Steward |
| Sig Arno | (stock footage from 'The Mummy's Hand') |
| Vinton Haworth | Frank, Reporter |
| Otto Hoffman | Retiring Cemetery Caretaker |
| Dick Hogan | Boy in Car |
| Cecil Kellaway | (stock footage from 'The Mummy's Hand') |
| Lew Kelly | Bartender |
| Rex Lease | Townsman |
| Charles Marsh | Man |
| Patrick McVey | Jake Lovell, New York Record |
| Leon Belasco | (stock footage from 'The Mummy's Hand') |
| William Ruhl | Nick |
| Glenn Strange | Man Riding Buckboard |
| Charles Trowbridge | (stock footage from 'The Mummy's Hand') |
| Tom Tyler | (stock footage from 'The Mummy's Hand') |
| Guy Usher | Doctor |
| Eddy Waller | Police Scientist |
| Walter Byron | Searcher |
| Harry Cording | Vic, a farmer |
| Mira McKinney | Vic's Wife |
| Peggy Moran | (stock footage from 'The Mummy's Hand') |
| Janet Shaw | Girl in Car |
| Grace Cunard | Farmer's Wife |
| Fern Emmett | Laura, wedding dress seamstress |
| Dick Foran | Steve Banning |
| Peggy Moran | Marta Solvani, Stage Name of Marta Sullivan |
| Wallace Ford | Babe Jenson |
| Eduardo Ciannelli | The High Priest |
| George Zucco | Professor Andoheb, High Priest of Karnak |
| Cecil Kellaway | The Great Solvani, Stage Name of Tim Sullivan |
| Charles Trowbridge | Dr. Petrie of the Cairo Museum |
| Tom Tyler | Kharis, the Mummy |
| Sig Arno | The Beggar |
| Eddie Foster | Egyptian Starting Fight |
| Harry Stubbs | Bartender |
| Michael Mark | Bazaar Owner |
| Mara Tartar | Girl Vendor |
| Leon Belasco | Ali, Foreman of Digging Crew |
| Jerry Frank | Egyptian Thug |
| Frank Lackteen | Temple Priest |
| Murdock MacQuarrie | Temple Priest |
| Ken Terrell | Egyptian Thug |
| John Carradine | Yousef Bey |
| Robert Lowery | Tom Hervey |
| Ramsay Ames | Amina Mansouri/Ananka |
| Barton MacLane | Inspector Walgreen |
| George Zucco | Andoheb, High Priest of Arkan |
| Frank Reicher | Prof. Matthew Norman |
| Harry Shannon | Sheriff Elwood |
| Emmett Vogan | Coroner |
| Lester Sharpe | Dr. Ayad, Scripps Museum |
| Claire Whitney | Mrs. Ella Norman |
| Oscar O'Shea | Scripps Museum Watchman |
| Lon Chaney Jr | .Kharis |
| Steve Barclay | Tom's Classmate |
| Jack Rockwell | Second Deputy |
| Jack C. Smith | Deputy Joe |
| Pietro Sosso | Priest |
| Ivan Triesault | Scripps Museum Guide |
| Carl Vernell | Student |
| Eddy Waller | Ben Evans |
| Anthony Warde | Detective at Museum |
| David Bruce | Radio Announcer |
| Fay Holderness | Woman |
| Mira McKinney | Mrs. Martha Evans |
| Dorothy Vaughan | Mrs. Ada Blake |
| Martha Vickers | Miss McLean, a student |
| Caroline Cooke | Townswoman |
| Bess Flowers | Scared Pedestrian |
| Lon Chaney Jr | .Kharis |
| Peter Coe | Dr. Ilzor Zandaab |
| Virginia Christine | Princess Ananka |
| Kay Harding | Betty Walsh |
| Dennis Moore | Dr. James Halsey |
| Martin Kosleck | Ragheb |
| Kurt Katch | Cajun Joe |
| Addison Richards | Pat Walsh |
| Holmes Herbert | Dr. Cooper |
| Charles Stevens | Achilles |
| William Farnum | Michael, the Sacristan |
| Napoleon Simpson | Goobie |
| James Crane | The Pharoah in flashback |
| Herbert Heywood | Hill |
| Jack Lorenz | Bulldozer driver |
| Tony Santaro | Ulysse, Berthe's Husband |
| Hector Sarno | Canjun in Cafe |
| Tom Tyler | Kharis in flashback |
| Budd Buster | Cajun with pipe at bar |
| Eddie Abdo | Pierre |
| Nina Bara | Young Cajun woman in Cafe |
| Ann Codee | Tante Berthe |
