| Robert Donat | Richard Hannay/Mr. Hammond/Capt. Fraser/Henry Hopkinson |
| Madeleine Carroll | Pamela/Mrs. Henry Hopkinson |
| Lucie Mannheim | Miss Annabella Smith |
| Godfrey Tearle | Professor Jordan |
| Peggy Ashcroft | Margaret, crofter's wife |
| John Laurie | John, crofter |
| Helen Haye | Mrs. Louisa Jordan |
| Frank Cellier | Sheriff Watson |
| Wylie Watson | Mr. Memory |
| Gus McNaughton | Commercial Traveller on Flying Scotsman |
| Jerry Verno | Commercial Traveller on Flying Scotsman |
| Peggy Simpson | Maid |
| Pat Hagate | M.C. Who Introduces Mr Memory |
| Alfred Hitchcock | Littering man near music hall |
| James Knight | Detective At London Palladium |
| Ivor Barnard | Political Meeting Chairman |
| Miles Malleson | Theatre manager |
| Frederick Piper | The Milkman |
| John Turnbull | Scottish Police Inspector |
| S.J. Warmington | Scotland Yard Man |
| Elizabeth Inglis | Professor Jordan's daughter |
| Hilda Trevelyan | Innkeeper's Wife |
| John Gielgud | Edgar Brodie/Richard Ashenden |
| Peter Lorre | The General |
| Madeleine Carroll | Elsa Carrington |
| Robert Young | Robert Marvin |
| Percy Marmont | Caypor |
| Florence Kahn | Mrs. Caypor |
| Charles Carson | 'R' |
| Lilli Palmer | Lilli |
| Tom Helmore | Colonel Anderson |
| Andreas Malandrinos | Manager |
| Howard Marion-Crawford | Karl (Lillis' Fiancee) |
| Michael Redgrave | Army captain |
| Michael Rennie | Bit Part |
| Michel Saint-Denis | Coachman |
| Edmund Gwenn | Mr. Hornblower |
| Helen Haye | Mrs. Amy Hillcrist |
| C.V. France | Squire John Hillcrist |
| Jill Esmond | Jill Hillcrist |
| Phyllis Konstam | Chloe Hornblower |
| John Longden | Charles Hornblower |
| Frank Lawton | Rolf Hornblower |
| Herbert Ross | Mr. Jackman |
| Dora Gregory | Mrs. Jackman |
| Edward Chapman | Dawker |
| R.E. Jeffrey | The Man in the Leather Coat |
| George Bancroft | The Man Who Employed Chloe |
| Ronald Frankau | Auctioneer |
| Ivor Barnard | Bit Part |
| Nova Pilbeam | Erica Burgoyne |
| Derrick De Marney | Robert Tisdall |
| Percy Marmont | Col. Burgoyne, Chief Constable |
| Edward Rigby | Old Will the China Mender |
| Mary Clare | Erica's Aunt Margaret |
| John Longden | Det. Insp. Kent |
| George Curzon | Guy |
| Basil Radford | Erica's Uncle Basil |
| Pamela Carme | Christine Clay |
| George Merritt | Detective Sergeant Miller |
| J.H. Roberts | Henry Briggs, Solicitor |
| Jerry Verno | Lorry driver at Tom's Hat cafe |
| H.F. Maltby | Police Sergeant Ruddock |
| John Miller | Police Constable |
| Syd Crossley | Policeman |
| William Fazan | Bit Part |
| Gerry Fitzgerald | Singer |
| Richard George | Policeman |
| Frank Atkinson | Petrol Pump Attendant |
| Alfred Hitchcock | Reporter/Photographer outside Magistrates Court |
| Mike Johnson | Tramp at Tom's Hat Cafe |
| Clive Baxter | Burgoyne Boy |
| Fred O'Donovan | Bit Part |
| Frederick Piper | Bit Part |
| Bill Shine | Serving at Tom's Hat cafe |
| Torin Thatcher | Nobby's Lodging House Caretaker |
| Jack Vyvian | Police Constable At Grand Hotel |
| Humberston Wright | Bit Part |
| Albert Chevalier | Bit Part |
| Anna Konstam | Bathing Girl |
| Pamela Bevan | Bit Part |
| Peggy Simpson | Bathing Girl |
| Beatrice Varley | Accused Mans Wife In First Court Case |
| Alfred Hitchcock | Himself - Host |
| Charles Laughton | Sir Humphrey Pengallan |
| Horace Hodges | Chadwick (Sir Humphrey's butler) |
| Hay Petrie | Sam (Sir Humphrey's groom) |
| Frederick Piper | Davis (Sir Humphrey's agent) |
| Herbert Lomas | Sir Humphrey's tenant |
| Clare Greet | Mrs. Marney (Sir Humphrey's tenant) |
| William Devlin | Sir Humphrey's tenant |
| Jeanne De Casalis | Sir Humphrey's friend |
| Mabel Terry-Lewis | Sir Humphrey's friend |
| A. Bromley Davenport | Sir Humphrey's friend |
| George Curzon | Sir Humphrey's friend |
| Basil Radford | Sir Humphrey's friend |
| Leslie Banks | Joss Merlyn |
| Marie Ney | Patience Merlyn (Joss' wife)) |
| Maureen O'Hara | Mary (Joss' niece) |
| Emlyn Williams | Harry (the peddler) |
| Wylie Watson | Salvation Watkins |
| Morland Graham | Sea Lawyer Sydney |
| Edwin Greenwood | Dandy |
| Mervyn Johns | Thomas |
| Stephen Haggard | The boy |
| Robert Newton | James 'Jem' Trehearne |
| William Fazan | Bit Part |
| Archie Harradine | Bit Part |
| Robert Adair | Capt. Murray |
| Harry Lane | Bit Part |
| Sam Lee | Bit Part |
| Alan Lewis | Bit Part |
| John Longden | Capt. Johnson |
| Aubrey Mather | Coachman |
| Philip Ray | Bit Part |
| Peter Scott | Bit Part |
| A. George Smith | Bit Part |
| O.B. Clarence | Coach passenger |
| Marie Ault | Coach Passenger |
| Mary Jerrold | Bit Part |
| Carl Brisson | Pete Quilliam |
| Malcolm Keen | Philip Christian |
| Anny Ondra | Kate Cregeen |
| Randle Ayrton | Caesar Cregeen |
| Clare Greet | Mother |
| Kim Peacock | Ross Christian |
| Wilfred Shine | Doctor |
| Harry Terry | Man |
| Nellie Richards | Wardress |
